RNA interference in Tribolium
I discussed the theory of RNA interference here.
In short, RNA interference induces the knock down of a gene by injecting double stranded RNA. Here I will show how we inject this double stranded RNA in Tribolium. We inject the RNA into pupa, this while they are easy to handle. However, it is also possible to inject RNA into the eggs, larva or adults.
First we have to gather the pupa by sieving them out of the flour. We are looking for pupa that are almost finished developing and are ready to hatch as adults. We can recognize them by their darkened wings and dark/red mouthparts (red arrows). After we collected pupa of the right stage we determine their sex. We do this because only females produce eggs and we are trying to discover the function of a gene in the egg.
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